Key features and details | |
Cat. No. | MABL-233 |
Name | Anti-Oligodendrocyte-specific surface antigen mAbs |
Clone No. | AFD-SCH94.03 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ICC, WB, ELISA, FC, IHC |
Species Reactivity | Rat, Human, Mouse |
Basic Information | |
Specificity | This antibody recognized an antigenic epitope found on the surface of oligodendrocytes. This antibody reacts with all major organs including the brain, spinal cord, heart, liver, kidney, stomach, and small intestine |
Alternative Name | oligodendrocyte; SCH 94.03 |
UniProt | |
Immunogen | The original antibody was generated by immunizing SJL/J mice with spinal chord homogenate (SCH) in incomplete Freund's adjuvant. |
Application Notes | The original IgM version of this antibody was found to promote remyelination of the central nervous system when passively transferred to SJL/J mice chronically infected with Theiler's murine encephalomyelitis virus (TMEV). The screening of the hybridomas for significant binding towards SCH was done using ELISA. Immunohistochemistry using this antibody showed multiorgan autoreactivity, and that it recognized both surface and cytoplasmic determinants on glial cells. The characterization of the antigen recognized by this antibody, various cell lines derived from glial (rat C6, mouse G26-20, human U373MG and U87MG), neural (human neuroblastoma), fibroblast (mouse L and 3T3), epithelial (human SCC-9 carcinoma), and lymphocytic (mouse CTLL2) origin were stained. |
Antibody First Published | Miller et al. Monoclonal autoantibodies promote central nervous system repair in an animal model of multiple sclerosis. J Neurosci. 1994 Oct;14(10):6230-8. PMID:7931575 |
Note on publication | Describes the potential therapeutic usefulness of this antibody in the treatment of CNS demyelinating diseases. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
