Key features and details | |
Cat. No. | MABL-234 |
Name | Anti-Phospho-Tau mAbs |
Clone No. | AFD-pT231/pS235_1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | SPR, ELISA, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody shows binding affinity towards pT231/pS235 peptide of the human tau protein. The binding affinity and specificity of this antibody relied heavily on the phosphorylation at Thr-231. |
Alternative Name | MAPT; TAU; PHF-tau; PHF; MAPTL; MTBT1; Tau proteins; Phospho-Tau (pT231/pS235); Microtubule‐associated protein tau; Neurofibrillary tangle protein; Paired helical filament-tau |
UniProt | P10636 |
Immunogen | |
Application Notes | ELISA was used for the screening of positive clones that bind phosphopeptides. The binding affinity of the IgG antibody towards pT231/pS235 phosphopeptide was found to be Kd = 0.35 nM using surface plasmon resonance. This antibody can recognize the phospho-tau epitopes in full-length human tau. This antibody was also used for the immunohistochemical analysis of the human brain with Alzheimer's disease (PMID: 23148212). |
Antibody First Published | Shih et al. An ultra-specific avian antibody to phosphorylated tau protein reveals a unique mechanism for phosphoepitope recognition. J Biol Chem. 2012 Dec 28;287(53):44425-34. PMID:23148212 |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
