Key features and details | |
Cat. No. | MABL-290 |
Name | Anti-17-Hydroxyprogesterone mAbs |
Clone No. | AFD-101.B11.1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | EIA, ELISA |
Species Reactivity | n/a |
Basic Information | |
Specificity | The antibody binds to 17-OHP with a Kd of 2.9 nM reported for the original antibody. |
Alternative Name | 17-OHP; 17alpha-hydroxyprogesterone |
UniProt | |
Immunogen | Mouse 17-OHP. |
Application Notes | The antibody binds specifically to 17-OHP, an endogenous progestogen which acts as a chemical intermediate in the synthesis of steroid hormones such as corticosteroids, androgens and estrogens. 17-OHP levels are extremely high in patients who suffer from congenital adrenal hyperplasia (CAH), a disorder of the adrenal glands caused by the deficiency of 21-hydroxylase which catalyses the production of cortisol. This results in the accumulation of the cortisol precursors, and in particular of 17- OHP. Monoclonal antibodies that bind specifically to 17-OHP can therefore be used to measure 17-OHP levels in the diagnosis of CAH. |
Antibody First Published | Sawada J, Terao T, Itoh S, Maeda M, Tsuji A, Hosoda H, Nambara T. Production and characterization of monoclonal antibodies to 17 alpha-hydroxyprogesterone. J Steroid Biochem. 1987 Oct;28(4):405-10. PMID:3312820 |
Note on publication | Describes the production of hybridoma clones producing antibodies to 17-OHP and the establishment of cross-reactivity with other steroids. A micro-EIA system of 17-OHP using the monoclonal antibodies can be used for the mass-screening of congenital adrenal hyperplasia. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
