Anti-TWEAK mAbs

Cat. No.

MABL-3158

Name

Anti-TWEAK mAbs

Clone No.

AFD- MTW-1

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Blocking, FC

Species Reactivity

Mouse

Basic Information

Specificity

This antibody is specific for murine TWEAK. It   has been shown not to react with TNF-α, FasL, and TRAIL.

Alternative Name

CD255; TNFSF12; TNF-related weak inducer of   apoptosis; MTW1; MTW 1; Tumor necrosis factor ligand superfamily member 12

UniProt

O54907

Immunogen

This antibody was raised by immunising SD   rats with CD8-mTWEAK, a fusion of the human CD8alpha extracellular region to   murine TWEAK.

Application Notes

The specificity of this antibody has been   confirmed in flow cytometric analysis, where it reacts specifically with   mTWEAK-transfected L5178Y cells, and not with parental L5178Y cells or cells   transfected with related TNF family members (Nakayama et al, 2003).   Additionally, this antibody has been used to assess TWEAK cell surface   expression in flow cytometric analysis (Nakayama et al, 2003). This antibody   has been shown to block CD8-mTWEAK cytotoxicity against L5178Y cells   over-expressing murine Fn14, the TWEAK receptor (Nakayama et al, 2003). When   administered to collagen-induced arthritis (CIA) mice, this blocking antibody   has been shown to have anti-inflammatory and antiangiogenic effects, with   associated improvements to both the clinical and histological manifestations   of CIA (Kamata et al, 2006). Additionally, administration of this antibody to   denervated mice attenuates denervation-induced skeletal muscle loss (Mittal   et al, 2010). Finally, administration of this antibody to CD163-/- mice subject to hind limb ischaemia inhibits the increased perfusion resulting   from CD163 deficiency (Akahori et al, 2015).

Antibody First   Published

Nakayama et al. Characterization of murine TWEAK and its receptor (Fn14) by monoclonal antibodies. Biochem Biophys Res Commun. 2003 Jul 11;306(4):819-25. PMID:12821115

Note on publication

Describes the original generation of this   antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.