Key features and details | |
Cat. No. | MABL-3181 |
Name | Anti-Vaginolysin mAbs |
Clone No. | AFD- 9B4 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, Block, ELISA |
Species Reactivity | G. vaginalis |
Basic Information | |
Specificity | The antibody binds to VLY with an affinity (Kd) of 0.29 nM and an IC50 of 0.067 nM (determined by hemolytic assay). The antibody has a strong neutralizing capability. |
Alternative Name | VLY |
UniProt | |
Immunogen | Recombinant VLY. |
Application Notes | The antibody binds specifically to VLY, a pore-forming toxic member of the cholesterol- dependent cytolysins, produced by the bacterium Gardnerella vaginalis. Once released, VLY acts by binding to cholesterol-rich membranes and forming transmembrane pores that result in lysis of the cell. The bacterium G. vaginalis is predominant in the vaginal tract of women with bacterial vaginosis (BV). Here releases VLY toxins that cause cytolysis and tissue destruction, and potentially leading to serious adverse effects such as infertility. Antibodies that are able to neutralize the toxic activity of VLY can be used as therapeutic agents for the treatment of BV. The antibody 9B4 has the strongest neutralizing activity, and binds to an epitope region comprising of the amino acids 122 to 268 of VLY. |
Antibody First Published | Zvirbliene A, Pleckaityte M, Lasickiene R, Kucinskaite-Kodze I, Zvirblis G. Production and characterization of monoclonal antibodies against vaginolysin: mapping of a region critical for its cytolytic activity. Toxicon. 2010 Aug 1;56(1):19-28. PMID:20298711 |
Note on publication | Describes the generation of two monoclonal antibodies with neutralizing activity against vaginolysin, the causative agent of bacterial vaginosis. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
