Anti-Variant surface glycoprotein mAbs

Cat. No.

MABL-3182

Name

Anti-Variant surface glycoprotein mAbs

Clone No.

AFD-cAbAn33

From

Recombinant Antibody

Isotype

Engineer antibody

Application

crystallization, SPR,   ELISA, FC

Species Reactivity

Trypanosoma

Basic Information

Specificity

The antibody is specific   for the conserved carbohydrate moiety on variant surface glycoprotein of the   trypanosome coat. The antibody cross-reacts with other VSGs (AnTat 1.1, MITat   1.1, MITat 1.5, and deglycosilated AnTat 1.1).

Alternative Name

VSG; cAb-An33; cAbAn33_1-0-0; VSG 221; Variant surface glycoprotein MITAT 1.2

UniProt

P26332

Immunogen

The antibody was   generated by immunizing a dromedary (Camelus dromedarius) with VSG purified   from T. brucei brucei AnTat 1.1. A VHH library was constructed and the   antibody was isolated by panning against immobilized AnTat 1.1.

Application Notes

The antibody specificity   and possible cross-reactivity with other VSGs (AnTat 1.1, MITat 1.1, MITat   1.5, and deglycosilated AnTat 1.1) was analyzed via solid-phase ELISA. The antibody recognized all the VSGs. The capacity of cAb-An33 to recognize its   epitope on living trypanosomes (AnTat 1.1, MITat 1.1, or MITat 1.5) was   analyzed via flow cytometry and microscopy. Flow cytometry showed that the antibody stains parasites of the three VSG classes, although not to the same intensity. Finally, the antibody was fused together with beta-lactamase and   employed as a recognition unit in immuno-toxins for trypanosomosis therapy   (Stijlemans et al., 2004; PMID: 14527957). The crystal structure of the VHH   fragment was determined. The humanized version of the antibody was constructed and the crystal structure determined.

Antibody First   Published

Stijlemans et al. Efficient targeting of conserved cryptic epitopes of infectious agents by  single domain antibodies. African trypanosomes as paradigm J Biol Chem. 2004   Jan 9;279(2):1256-61. PMID:14527957

Note on publication

The original paper describes the generation and characterization of the antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.