Key features and details | |
Cat. No. | MABL-3183 |
Name | Anti-VASA protein mAbs |
Clone No. | AFD-1E 9 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for the C-terminal domain of human VASA protein. The binding epitope comprises amino acids 712-721 (SQAPNPVDDE) of the human VASA protein. VASA homolog is also known as ATP-dependent RNA helicase DDX4, which is required during spermatogenesis. VASA protein is present in fetal and adult gonadal germ cells in both males and females and is most abundant in spermatocytes and mature oocytes. |
Alternative Name | DDX4;VASA; VASA-1; Probable ATP-dependent RNA helicase DDX4; DEAD box protein 4; Vasa homolog; 1E9/VH1E3 |
UniProt | Q9NQI0 |
Immunogen | The original antibody was isolated from an scFv phage display library generated from the pool of normal human B cells from 40 blood donors. The particular antibody was isolated by panning the library against human VASA-1 peptide (amino acids 712-721). Later on the scFv antibody was converted to an IgG. |
Application Notes | The binding characterization of this antibody to human VASA protein and VASA peptides (residues 700-724; residues 712-721; residues 700-709) was done using ELISA. It was found that this antibody was specific for VASA peptide with residues 712-721. The IgG antibody binds human VASA protein in a dose dependent manner with an EC50 of 0.08919 nM (US20180155445). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
