Anti-VCAM-1 mAbs

Cat. No.

MABL-3186

Name

Anti-VCAM-1 mAbs

Clone No.

AFD- 6C7.1

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Blocking, therapeutic, FC, IF, IHC

Species Reactivity

Mouse

Basic Information

Specificity

This antibody is specific for murine VCAM-1.   VCAM-1 is an important in cell-cell recognition. Appears to function in   leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1   (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal   transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic   role both in immune responses and in leukocyte emigration to sites of   inflammation.

Alternative Name

Vascular cell adhesion protein 1; Vcam1;   V-CAM 1; CD106

UniProt

P29533

Immunogen

This antibody was rasied against VCAM-1.

Application Notes

The original rat version of this antibody   was used to treat experimental autoimmune encephalomyelitis (EAE in female   SJL/N mice). It resulted in a later onset of tEAE (Engelhardt et al, 1998; pmid:9854045). To asses α4-integrin–mediated T cell interaction with VCAM-1 on blood-brain barrier–endothelium in experimental autoimmune   encephalomyelitis flow cytometry was preformed on T cell blasts labeled with   the rat version of this antibody (Vajkoczy et al, 2001; pmid:11518729). Futhermore, PLP- specific T lymphoblasts were used for immunofluorescence with the rat version of this antibody (Vajkoczy et al, 2001; pmid:11518729). And finally, mice were injected with the rat version of this antibody to   asses how it can slow down progression of EAE. It showed an ability to slow   EAE down (Vajkoczy et al, 2001; pmid:11518729).

Antibody First   Published

Engelhardt et al. The development of experimental autoimmune encephalomyelitis in the mouse requires   alpha4-integrin but not alpha4beta7-integrin J Clin Invest. 1998 Dec   15;102(12):2096-105. PMID:9854045

Note on publication

The effects of mAb therapy on the development of EAE in the SJL/N mouse was compared, using a large panel of   mAbs directed against alpha4, beta7, the alpha4beta7-heterodimer, and against   VCAM-1. Although encephalitogenic T cells express both alpha4-integrins, mAbs   directed against the alpha4beta7-heterodimer or against the beta7-subunit did   not interfere with the development of EAE.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.