Anti-CD156b mAbs

Cat. No.

MABL-734

Name

Anti-CD156b mAbs

Clone No.

AFD- MEDI3622

From

Recombinant Antibody

Isotype

Engineer antibody

Application

in vitro, in vivo, SPR, ELISA, FC

Species Reactivity

Human

Basic Information

Specificity

This atibody is specific for a unique surface   loop of sIVa-sIVb β-hairpin on ADAM17.

Alternative Name

CSVP; TACE; ADAM17; ADAM 17; Snake   venom-like protease; TNF-alpha convertase; TNF-alpha-converting enzyme; Disintegrin and metalloproteinase domain-containing protein 17

UniProt

P78536

Immunogen

This antibody was raised by panning the CAT   library against ADAM17.

Application Notes

The original antibody was used for flow   cytometry on different variants of ADAM17 (Peng et al., 2016; PMID：27610476), and on   T4-2 cells (Rios-Doria et al., 2015; PMID：25948294). This antibody was used for surface plasmon resonance on   ADAM17. This antibody was used for biolayer inferometry on ADAM17 (Peng et   al., 2016; PMID：27610476). This antibody was used in vivo on mice with implanted   tumors, it showed the ability to strongly decrease the tumor growth and   volume. The influence of this antibody on TNF alpha shedding was assessed, it   inhibited the shedding. A similar experiment was also perform for EGFR ligand   shedding and yielded similar results. This antibody was used for ELISA on   ADAM17 (Rios-Doria et al., 2015; PMID：25948294). This antibody was added to NK cells and NK92 cells   stimulated with different tumor cell lines, it showed that the antibody   blocks ADAM17 activity in NK cells but not the activation of ADAM17. The   antibody was also shown to enhance IFN gamma production in NK cells (Mishra   et al., 2018; PMID：29978334).

Antibody First Published

Rios-Doria et al. A Monoclonal Antibody to   ADAM17 Inhibits Tumor Growth by Inhibiting EGFR and Non-EGFR-Mediated   Pathways Mol Cancer Ther. 2015 Jul;14(7)：1637-49.PMID:25948294

Note on publication

The original paper reports the discovery of  a potent and specific ADAM17 inhibitory antibody, MEDI3622, which induces tumor regression or stasis in many EGFR-dependent tumor models.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.