Cat. No.
MABL-281
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Zinnia elegans
Clone No.
XD3
From
Recombinant Antibody
Specificity
This antibody recognises (1→4)-β-d-galactan. The epitope recognised is present on the primary wall of tracheary-element precursor cells, but absent from mature tracheary elements.
Alternative Names
14bdGal; (1→4)-β-d-galactan
UniProt
Immunogen
This antibody was isolated from a phage-display library raised against a cell wall fraction of Zinnia elegans xylogenic culture cells. Cell wall fractions were isolated from Zinnia mesophyll cells that had been cultured either in a differentiation-inducti
Application Notes
Whole-cell ELISA against cultured plant cells has confirmed that this antibody is
differentiation-specific, as the epitope recognised by this antibody increased specifically on cells cultured in a differentiation-inductive medium but not on those cultured in a noninductive medium (Shinohara et al, 2002).Equally, this antibody has been shown in ELISA analysis to recognise (1→4)-β-d-galactan in a pectic fraction (Shinohara et al, 2015).
Antibody First Published
Shinohara et al. Isolation of monoclonal antibodies recognizing rare and dominant epitopes in plant vascular cell walls by phage display subtraction J Immunol Methods. 2002 Jun 1;264(1-2):187-94. PMID:12191521
Note on publication
Describes the original generation of this antibody, and its characterisation in ELISA
analysis.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
