Cat. No.
MABL-287
Application
funtional assay, RIA, ELISA
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
4B5
From
Recombinant Antibody
Specificity
This antibody is specific for 14G2a. 14G2a is a mouse IgG2a antibody specific for human GD2.
Alternative Names
antibody 14G2a; ganglioside; ganglioside 2a
UniProt
Immunogen
The original human version of this antibody was generated by treating humans with the 14G2a antibody then MNCs were harvested and hybridized with the murine myeloma cell line P3X.Ag8.653.
Application Notes
To determine the functionality of the mouse version of this antibody, an ELISA was performed to test if this antibody was able to bind to antigen 14G2A. Furthermore, the antibody was also tested in a western blot on antigen 14G2A (EP1000082A2). A radioimmunoassay was performed to screen for anti-14G2a activity of the human version of this antibody. To assess the ability of the human version of this antibody to mediate a cellular immune response. Rabbits were immunized with 4B5. Then, irradiated Mel-21 cells were implanted. The result was induration at the site of the irradiated cells (Saleh et al., 1993; PMID: 8376782).
Antibody First Published
Saleh et al. Generation of a human anti-idiotypic antibody that mimics the GD2 antigen J Immunol. 1993 Sep 15;151(6):3390-8. PMID:837678
Note on publication
Four human anti-14G2a secreting hybridomas were generated and the mAb product of one of the hybridomas was characterized.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
