Cat. No.
MABL-290
Application
EIA, ELISA
Isotype
Engineer antibody
Species Reactivity
n/a
Clone No.
101.B11.1
From
Recombinant Antibody
Specificity
The antibody binds to 17-OHP with a Kd of 2.9 nM reported for the original antibody.
Alternative Names
17-OHP; 17alpha-hydroxyprogesterone
UniProt
Immunogen
Mouse 17-OHP.
Application Notes
The antibody binds specifically to 17-OHP, an endogenous progestogen which acts as a chemical intermediate in the synthesis of steroid hormones such as corticosteroids, androgens and estrogens. 17-OHP levels are extremely high in patients who suffer from congenital adrenal hyperplasia (CAH), a disorder of the adrenal glands caused by the deficiency of 21-hydroxylase which catalyses the production of cortisol. This results in the accumulation of the cortisol precursors, and in particular of 17- OHP. Monoclonal antibodies that bind specifically to 17-OHP can therefore be used to measure 17-OHP levels in the diagnosis of CAH.
Antibody First Published
Sawada J, Terao T, Itoh S, Maeda M, Tsuji A, Hosoda H, Nambara T. Production and characterization of monoclonal antibodies to 17 alpha-hydroxyprogesterone. J Steroid Biochem. 1987 Oct;28(4):405-10. PMID:3312820
Note on publication
Describes the production of hybridoma clones producing antibodies to 17-OHP and the establishment of cross-reactivity with other steroids. A micro-EIA system of 17-OHP using the monoclonal antibodies can be used for the mass-screening of congenital adrenal hyperplasia.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
