Cat. No.
MABL-294
Application
IP, RIA, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
10G6D6
From
Recombinant Antibody
Specificity
This antibody binds specifically to 17β estradiol, a mammalian estrogenic steroid hormone that is produced in the ovaries, placenta, and testis.
Alternative Names
17-beta-estradiol; E2; ethynyl estradiol; oestradiol
UniProt
Immunogen
estradiol-6-ethyl methoxy carbonyl (EMC)–bovine serum albumin (BSA).
Application Notes
This antibody binds 17ß estradiol with a sensitivity of 33 ± 9 pg/ml. In women, 17β estradiol plays an important role in the development of secondary sexual characteristics and the maturation of long bones. It is also responsible for the activation of certain oncogenes and can be involved in the development of breast cancer and cancer of the uterine lining. Anti-estradiol antibodies can be useful in the detection of hormone concentrations in serum samples. This can be used therapeutically to monitor follicular maturation in cases of female infertility and estradiol-secreting tumors.
Antibody First Published
Bettsworth F, Monnet C, Watelet B, Battail-Poirot N, Gilquin B, Jolivet M, Menez A, Arnaud M, Ducancel F. Functional characterization of two anti-estradiol antibodies as deduced from modelling and site-directed mutagenesis experiments. J Mol Recognit. 2001 Mar-Apr;14(2):99-109. PMID:11301480
Note on publication
Describes the role of monoclonal antibodies in measuring concentrations of steroid hormones in human serum samples, and the characterization of two anti-estradiol monoclonal antibodies for this use.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
