Cat. No.
MABL-301
Application
SPR, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
S73-2
From
Recombinant Antibody
Specificity
This antibody is specific for 2.4[2.8]2.4-linked Kdo tetrasaccharide. Which is a partial structure of chlamydial lipopolysaccharide (LPS).
Alternative Names
Kdo; alpha-Kdo; 3-deoxy-alpha-D-manno-oct-2-ulopyranosonic acid; 3- deoxy-alpha-D-manno-oct-2-ulopyranosonic acid; Chlamydia lipopolysaccharide (LPS) component
UniProt
Immunogen
This antibody was raised by immunizing mice with Kdo(2f8)[Kdo(2f4)]Kdo(2f4)Kdo(2f6)GlcNAc4P-(1f6)GlcNAcol-BSA and further immunizing them with
Kdo(2f8)[Kdo(2f4)]Kdo-BSA.
Application Notes
To screen hybridomas for this antibody ELISA was performed on neoglycoconjugates and immunofluorescence was performed on cells infected with either C. trachomatis or C. psittaci. Surface plasmon resonance was performed on Kdo(2f8)Kdo(2f4)KdoBSA (164, 1650, and 2460 RUs), Kdo(2f4)Kdo(2f4)Kdo-BSA, Kdo(2f4)Kdo BSA and BSA(Sigma), as a reference protein using the mouse version of this antibody (Brooks et al, 2010; pmid:20000757).
Antibody First Published
Brooks et al. Antibodies raised against chlamydial lipopolysaccharide antigens reveal convergence in germline gene usage and differential epitope recognition. Biochemistry. 2010 Jan 26;49(3):570-81. PMID:20000757
Note on publication
The structures of antigen-binding fragments from two related monoclonal antibodies have been determined to high resolution in the presence of several carbohydrate antigens raised against chlamydial lipopolysaccharide.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
