Cat. No.
MABL-321
Application
ICC, WB, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Rat
Clone No.
58K-9
From
Recombinant Antibody
Specificity
This antibody is specific for the 58k golgi protein.
Alternative Names
FTCD; 58K Golgi protein; 58K
UniProt
O88618
Immunogen
The original antibody was generated by immunizing a BALB/c mouse with purified 58K golgi.
Application Notes
The original version of this antibody was used for an immunofluorescence experiment to stain the golgi apparatus (Chen et al., 2006; PMID:16431922), in transfected CHO cells (Johne et al., 2008; PMID:18216281), in HSC8B cells (Mao et al., 2009; PMID: 19139721), in mouse melanocyte cells (Xu et al., 2010; PMID:20502676), in HeLa cells (Kotak et al., 2012; PMID:23027904), and in Communication_x0002_incompetent HeLa cells (Kleopa et al., 2006; PMID: 16476939). This antibody was used for a western blot to stain the golgi proteins on rat hepatic cells (Mao et al., 2009; PMID: 19139721), on human protein (Clemen et al., 2010; PMID:20833645).
Antibody First Published
Bloom and Brashear A Novel 58-,kDaProtein Associates with the Golgi Apparatus and Microtubules J Biol Chem. 1989 Sep 25;264(27):16083-92. PMID:2777777
Note on publication
The original paper identifies proteins involved in linking microtubules to other cytoplasmic structures.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
