Cat. No.
MABL-336
Application
crystallization, WB, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Chinese Hamster, Dog, Human
Clone No.
C219
From
Recombinant Antibody
Specificity
This antibody is specific for the epitope VQ(E/A)ALD of Chinese hamster ABCB1.
Alternative Names
CD243; P-glycoprotein 1; PGP1; PGY1; Multidrug resistance protein 1; Phospholipid transporter ABCB1; ATP-dependent translocase ABCB1; ATP-binding cassette sub-family B member 1
UniProt
P21448
Immunogen
The original antibody was raised against plasma membranes of multidrug-resistant Chinese hamster ovary cells.
Application Notes
The original antibody was used for a western blot on whole cell extracts of E. coli cells overexpressing a fusion of the carboxyl-terminal NBD of Pgp to maltose-binding protein. The structure of the original antibody was determined using x-ray crystallography (Hoedemaeker et al., 1997; PMID:9368049, van Den Elsen et al., 1999; PMID:10570132). This antibody was used for immunohistochemistry on breast cancer cell lines, to determine the cross reactivity of this antibody with p185. This antibody was used for an ELISA on different peptides from p170 and p185 (Liu et al., 1997; PMID:9337349). This antibody was used for immnohistochemistry on high-grade lymphoma from dogs (Bergman et al., 1996; PMID:8947866), and on Ovarian Epithelium, Fallopian Tube, and Ovarian Carcinoma Specimens (Finstad et al., 1991; PMID:1682363).
Antibody First Published
Finstad et al. Some monoclonal antibody reagents (C219 and JSB-1) to P_x0002_glycoprotein contain antibodies to blood group A carbohydrate determinants: a problem of quality control for immunohistochemical analysis J Histochem Cytochem. 1991 Dec;39(12):1603-10. PMID:1682363
Note on publication
The original study demonstrates that some commercial lots of these MAb, even those supplied as purified immunoglobulins, contain contaminating anti-A blood group antibodies.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
