Cat. No.
MABL-338
Application
RIA, ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
15-I-C5
From
Recombinant Antibody
Specificity
This antibody reacts specifically with physiologically active 2-cis-(S)-form of the endogenous plant growth regulator, abscisic acid. This antibody does not cross react with ABA-catabolites, phasic and dihydrophaseic acid, (R)-ABA, 2-trans-ABA, the ABA-conjugate, ABA-P-D-glucopyranosyl ester, putative ABA precursor and xanthoxin. Abscisic acid is an important sesquiterpenoid plant hormone which acts as a regulator of plant responses to environmental stresses including drought, soil salinity, cold tolerance, freezing tolerance, heat stress and heavy metal ion tolerance. It also plays a role in many plant developmental processes.
Alternative Names
ABA; ChEBI:2365; 16-I-C5
UniProt
Immunogen
The original antibody was generated by immunizing BALB/c mice with an antigen was which was synthesized by reacting 2-cis-(R, S)-ABA4’-tyrosylhydrazone with p-aminohippuric acid-substituted bovine serum albumin emulsified in an equal volume of complete Freund’s adjuvant.
Application Notes
The binding affinity and specificity of the original IgG1 antibody was determined using radioimmunoassay (Mertens et al., 1983). This antibody was also used in the generation of a highly sensitive enzyme amplified immunoassay to detect plant growth regulator abscisic acid in homogeneous tissue samples dissected from Vicia faba L. leaves (PMID: 16593922). This antibody was also used in a study to detect whether it can identify any abscisic acid analogs and it was found that this antibody did not cross-react with analogs with the ring double bond reduced and the C-2' methyl cis to the sidechain (PMID: 16667979). An scFv version of this antibody was generated using phage display technology. Specificity of this scFv antibody to abscisic acid was comparable to the binding properties of the complete IgG1 antibody which was verified by competition ELISA (Artsaenko et al., 1994; pmid:8528285). The binding kinetics of the scFv version and the whole IgG1 parental version of this was studied in PMID: 27023768.
Antibody First Published
Mertens et al. Monoclonal antibodies for the detection and quantitation of the endogenous plant growth regulator abscisic acid. FEBS Lett. (1983; 160: 269–272.PMID:
Note on publication
Describes the generation and characterization of two antibodies generated against plant hormone abscisic acid.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
