Cat. No.
MABL-362
Application
WB, ELISA, FC, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
GT1-mAb1
From
Recombinant Antibody
Specificity
This antibody is specific to EBP05419, an immunizing peptide with the sequence C_x0002_TGPPAKKAISELP. This peptide was used in the production of the polyclonal Goat Anti-AIF1 antibody from which this antibody is derived.
Alternative Names
AIF1; IBA1; allograft inflammatory factor 1; AIF-1; IRT-1;interferon gamma responsive transcript; ionized calcium-binding adapter molecule 1; Daintain; DADB-70P7.8; allograft inflammatory factor-1 splice variant Hara-1; IRT1; protein G1
UniProt
P55008
Immunogen
This antibody was obtained by sequencing a polyclonal goat IgG raised against a peptide with the sequence C-TGPPAKKAISELP.
Application Notes
This antibody (GT1-mAb1) was one of two monoclonal antibodies discovered by analyzing a polyclonal antibody (GT-1) sample. Briefly, de novo sequencing of GT-1 resulted in the full_x0002_length sequences of two distinct monoclonal antibodies (GT1-mAb1 and GT1-mAb2) in both framework and CDR regions. Both antibodies were recombinantly expressed by Absolute Antibody in HEK293 cells and tested in various assays. GT1-mAb1 was tested against the peptide antigen in an ELISA, and it showed activity comparable to that of the original polyclonal antibody. GT1-mAb1 was found to bind the target peptide in a non-competitive manner with GT1-mAb2 while being efficiently blocked by the original polyclonal antibody, proving that both monoclonal antibodies bind distinct epitopes and that GT1-mAb1 possess binding characteristics of the input polyclonal antibody. Furthermore, GT1-mAb1 was compatible
with immunofluorescence (IF), flow cytometry (FC), and Western blot (WB), with an FC signal observedly
more distinct than that of GT1-mAb2.
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
