Cat. No.
MABL-379
Application
ELISA, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
AB2
From
Recombinant Antibody
Specificity
This antibody binds human alpha-fetoprotein (AFP). AFP is made in the liver of developing babies. It binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. AFP levels in healthy adults is very low. Elevated levels of AFP in adult human blood is a marker for cancer.
Alternative Names
AFP; Alpha-fetoglobulin; A-Feto-protein
UniProt
P02771
Immunogen
The original antibody was generated by immunizing BALB/c mice with human alphafetoprotein.
Application Notes
This antibody can be used in combination with another anti-alpha fetoprotein antibody AB3 for detection of human AFP using sandwich ELISA. AB3 is used as the capture antibody and HRP labelled AB2 is used as detection antibody. This antibody was also used for the immunofluorescent staining of HepG2 hepatoma cells and immunohistochemical analysis of human liver cancer tissue (CN107022029).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
