Cat. No.
MABL-401
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
D51
From
Recombinant Antibody
Specificity
This antibody shows reactivity with amoxicillin, which is a penicillin antibiotic used to treat bacterial infections such as tonsillitis, bronchitis, sinusitis, pneumonia, and infections of the ear, nose, throat, skin, or urinary tract. This antibody shows some cross reactivity with aztreonam.
Alternative Names
AX; Amoxycillin; Amox; ChEBI：2676
UniProt
Immunogen
The original antibody was generated by immunizing Lama glama with amoxicillin conjugated with human serum albumin. The peripheral blood lymphocytes of the llama were used to generate a VHH library and the specific antibody was selected by panning the library against amoxicillin-KLH conjugate.
Application Notes
The binding characterization of this antibody was done using ELISA. This antibody in combination with an anti-omalizumab antibody E5 was used in the generation of a bispecific antibody for determination of allergy against the ampicillin antibiotic(PMID 34931798).
Antibody First Published
Segovia-de Los Santos et al. Bispecific Single-Domain Antibodies as Highly Standardized Synthetic Calibrators for Immunodiagnosis. Anal Chem. 2022 Jan 18;94(2) 1342-1349.PMID 34931798
Note on publication
Describes the generation and characterization of two bispecific calibrators with potential application for the diagnosis of allergy against the antibiotics aztreonam and amoxicillin in humans.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
