Cat. No.
MABL-422
Application
SPR, WB, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Plasmodium spp
Clone No.
F8.12.19
From
Recombinant Antibody
Specificity
This antibody recognizes a discontinuous conformational epitope located on domain III of the ectoplasmic region of the apical membrane antigen 1 (AMA1) of Plasmodium vivax and also cross reacts with AMA1 from Plasmodium knowlesi, Plasmodium cynomolgi, Plasmodium berghei and Plasmodium falciparum. AMA1 is involved in parasite invasion of erythrocytes.
Alternative Names
AMA1;AMA-1; Apical merozoite antigen 1;
UniProt
Q9BIM8; Q9TY14
Immunogen
The original antibody was generated by immunizing BALB/c mice with recombinant ectoplasmic region of AMA1 from Plasmodium vivax.
Application Notes
This antibody can recognize AMA1 of Plasmodium vivax, Plasmodium knowlesi, Plasmodium cynomolgi, Plasmodium berghei and Plasmodium falciparum in an immunofluorescence assay on mature schizonts. The binding region is a discontinuous epitope located on domain III of the ectoplasmic region, the major component being a loop containing a cystine knot. The binding of F8.12.19 to recombinant AMA1 from both P. vivax and P. falciparum was measured by surface plasmon resonance. This antibody shows no function-blocking effect was detected in ex vivo parasite growth inhibition assays. (PMID: 17229439). This antibody a dilution of 1 16,000 was used for the detection of recombinant PvAMA-1 and recombinant PvAMA-DII-III using dot-blot and ELISA assays (PMID: 28855657). This antibody was also used for the identification of PfAMA1 using western blot (PMID: 25236489).
Antibody First Published
Igonet et al. Cross-reactivity studies of an anti-Plasmodium vivax apical membrane antigen 1 monoclonal antibody binding and structural characterization. J Mol Biol. 2007 Mar 9;366(5):1523-37. PMID:17229439
Note on publication
This paper analysis the cross reactivity of an anti-AMA1 antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
