Cat. No.
MABL-428
Application
WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
MabB23
From
Recombinant Antibody
Specificity
This antibody binds human apolipoprotein B (APOB). Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. It is involved in the metabolism of lipids and is the main protein constituent of lipoproteins such as very low- density lipoprotein (VLDL) and low-density lipoprotein (LDL, the “bad cholesterol”).
Alternative Names
APOB; Apolipoprotein B-100; Apo B-100
UniProt
P04114
Immunogen
The original antibody was generated by immunizing female BALB/c mice intraperitoneally with 20ug of human apo B-100 and dead Salmonella microbes.
Application Notes
The binding characterization of this antibody towards human APO-B was done using ELISA and immunoblotting. This antibody was also used in a serum assay to determine the quantity of apo- B in a sample using competitive ELISA. This antibody was shown to react with isolated human Apo-B as well as low density lipids but not with high density lipids and lipoprotein-deficient serum (Yoon et al., 1993).
Antibody First Published
Yoon et al. Production of monoclonal antibodies to human apolipoprotein B- 100 and development of a monoclonal antibody-based, competitive enzyme-linked immunoassay for apolipoprotein B. Korean Journal of Lipidology, 1993, vol. 3, no. 2, pp. 229-239. PMID
Note on publication
Describes the generation and characterization of two antibodies against human apolipoprotein B-100.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
