Cat. No.
MABL-540
Application
WB, ELISA, FC, IF
Isotype
Engineer antibody
Species Reactivity
Burkholderia mallei
Clone No.
BpaB#4
From
Recombinant Antibody
Specificity
This antibody is specific for Burkholderia mallei autotransporter BpaB. Autotransporter proteins form one of the largest class of virulence factors in Gram-negative organisms and perform important functions in pathogenesis including flocculation, formation of biofilms , complement resistance, host cell adhesion and entry, intracellular motility and replication, cytotoxicity, and lipolytic activity.
Alternative Names
Burkholderia mallei BpaB autotransporter; BpaB-4; BpaB 4; BpaB- MAb#4; BpaB MAb#4; BpaB autotransporter of B. mallei
UniProt
Immunogen
This antibody was raised by immunising Balb/c mice with purified his-tagged BpaB .
Application Notes
Given the function of autotransporters, such as BpaB in pathogenesis and their overall structure, they are excellent targets for developing medical countermeasures (MCM) against pathogenic organisms. A significant portion of AT (passenger domain) is readily accessible for recognition by the immune system as it is exposed on the bacterial surface. Consequently, BpaB#4 antibody is recommended as a potential research target in treatment and biosafety measures associated with B. mallei. Efficient and specific binding of this antibody to BpaB was confirmed via Western blot; BpaB#4 (BpaB-MAb#4) identified this autotransporter in the material from whole cell lysates (WCL), total membrane proteins (TMP) and sarkosyl-insoluble fraction containing OM proteins (OMP) coming from recombinant E.coli with B. mallei BpaB (Zimmerman et al., 2015). BpaB was shown to be a promising target for the B. mallei therapies due to its involvement in adherence to host cells, biofilm formation and pathogenicity in a mouse model of glanders (Zimmerman et al., 2015). BpaB#4 antibody was further utilised in the identification of B. mallei via immunostaining in various tissues experimentally infected with the bacterium, expanding the antibody's potential use in therapeutics and diagnostics (Zimmerman et al., 2018).
Antibody First Published
Zimmerman et al. The Autotransporter BpaB Contributes to the Virulence of Burkholderia mallei in an Aerosol Model of Infection. PLoS One. 2015 May 20;10(5):e0126437. doi: 10.1371/journal.pone.0126437. eCollection 2015. PMID:25993100
Note on publication
This article describes the generation and characterisation of BpaB#4 antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
