Cat. No.
MABL-557
Application
functional assay, SPR, ELISA
Isotype
Engineer antibody
Species Reactivity
Brucella
Clone No.
YsT9.1
From
Recombinant Antibody
Specificity
This antibody is specific for the brucella class of bacteria. Brucella is a genus of Gram-negative bacteria, named after David Bruce (1855–1931). They are small (0.5 to 0.7 by 0.6 to 1.5 µm), nonencapsulated, nonmotile, facultatively intracellular coccobacilli.
Alternative Names
YsT9-1
UniProt
Immunogen
This antibody was raised by immunizing mice with B.melitensis.
Application Notes
To characterize the binding of this antibody, an ELISA was preformed on a B. melitensis 16M O-polysaccharide-biotin conjugate using the mouse version of this antibody (Bundle et al, 1989; pmid:2474505). An anti-idiotypic antibody was raised by panning against the mouse version of this antibody. Furthermore surface plasmon resonace was preformed using an anti-idiotipic antibody with the mouse version of this antibody as antigen (Tanha et al, 2002; pmid:12009207).
Antibody First Published
Bundle et al. Definition of Brucella A and M epitopes by monoclonal typing reagents and synthetic oligosaccharides. Infect Immun. 1989 Sep; 57(9): 2829–2836. PMID:2474505
Note on publication
The paradigm that Brucella A and M epitopes are simultaneously expressed on single cells and within one antigen molecule was reinvestigated by using polysaccharide-specific murine monoclonal antibodies.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
