Cat. No.
MABL-574
Application
Block, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
Fab 103
From
Recombinant Antibody
Specificity
Fab 103 binds only to C5a and not to C5. This antibody is likely to recognise a conformational epitope because it failed to bind C5a when it was linked to microtiter plates. The epitope is outside the active site of C5a.
Alternative Names
Complement Component 5a
UniProt
P01031
Immunogen
A panel of mAbs against the activated complement component C5a was obtained from a filamentous phage M13-Fab display library generated from mice immunized with human rC5a. Fabs isolated from the library after iterative selection against rC5a bound to both rC5a and purified C5. To isolate Fabs specific for neoepitopes expressed on C5a but not on the native complement component C5, the library was rescreened in a competitive manner. The phage Fab library was first incubated with immobilized C5 to deplete C5 reactive Fabs. The C5 nonadherent phage were then incubated with immobilized rC5a in the presence of soluble C5. Bound phage were eluted and subjected to two additional cycles of subtraction with immobilized C5 and selection with immobilized rC5a in the presence of soluble C5. After three cycles of this competitive biopanning, Fabs reactive with rC5a were isolated.
Application Notes
Fab 103 has been used in ELISAs in order to specifically detect C5a and quantify C5a in human CSF (Stahel 1997). It can be used to block C5a binding to its receptor. mAbs to C5a may have a therapeutic benefit in sepsis/ARDS.
Antibody First Published
Ames et al. Isolation of Neutralizing Anti-C5a Monoclonal Antibodies from a Filamentous Phage Monovalent Fab Display Library Journal of Immunology, 1994, 152： 4572. PMID：8157971
Note on publication
Describes the generation of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
