Cat. No.
MABL-594
Application
WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Cow, House, Human
Clone No.
2D1-E1-B12
From
Recombinant Antibody
Specificity
This antibody is specific for calf, human and mouse thymus ATPase A. Calf ATPaseA is a TP- dependent annealing helicase that binds selectively to fork DNA relative to ssDNA or dsDNA and catalyzes the rewinding of the stably unwound DNA.
Alternative Names
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1; HepA-related protein; Sucrose nonfermenting protein 2-like 1
UniProt
Q9TTA5
Immunogen
The mouse version of this antibody was generated by immunizing mice with immunopurified calf thymus ATPaseA.
Application Notes
The mouse version of this antibody was used for a western blot on calf DNA- dependent ATPase A isolated from calf thymus using immunoprecipitation (Mesner et al, 1993; pmid:8394114). The specificity of the mouse version of this antibody was confirmed using an ELISA on the ATPase A protein (Mesner et al, 1991; pmid:1720977).
Antibody First Published
Mesner et al. DNA-dependent adenosinetriphosphatase A is the eukaryotic analogue of the bacteriophage T4 gene 44 protein: immunological identity of DNA replication-associated ATPases Biochemistry. 1991 Dec 10;30(49):11490-4. PMID:1720977
Note on publication
Three stable murine hybridomas that secrete monoclonal antibodies which recognize calf thymus DNA-dependent adenosinetriphosphatase A were generated.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
