Cat. No.
MABL-604
Application
crystallography, HI, NTRL, RIA, ELISA
Isotype
Engineer antibody
Species Reactivity
canine parvovirus, feline panleukopenia virus, Mink enteritis virus, Raccoon parvovirus
Clone No.
MabB
From
Recombinant Antibody
Specificity
This antibody is specific for CPV-2's, FPV's capsid (site A). It also cross-reacts with MEV and RPV.
Alternative Names
Coat protein VP1; CPV-2; FPV; MEV; RPV; ABCD_AD539
UniProt
P17455; P04864
Immunogen
The original antibody was generated by immunizing a Lewis-strain rat with purified infectious (heavy) FPV-b.
Application Notes
The original version of this antibody (rat IgG2b) was raised and tested against CPA, FPV, and MEV and was found to inhibit and neutralize CPV, FPV, and MEV-a and -b in neutralization and HI assays. It was also used in RIAs. In the HI test, it was found to inhibit CPV-a;-b;-c-;-d; and -e, FPV-a; -b; -c, RPV-a; -b; -c; -d; and -e, and MEV-a; -b; -c; -d; -e; -f; -g; and -h. In the RIA, it was found to react with CPV-d and FPV-b (Parrish and Carmichael, 1983; PMID: 6194613). The Fab version of this antibody was used in a cryoEM analysis of capsid:Fab complexes, and its Kd was estimated via ELISA as ~6.1 × 10-9 M (Nelson et al., 2007; PMID: 17217977). The Fab version of this antibody was crystallized, and its structure was determined (Hafenstein et al., 2009; PMID: 19321620).
Antibody First Published
Parrish & Carmichael Antigenic structure and variation of canine parvovirus type-2, feline panleukopenia virus, and mink enteritis virus. Virology. 1983 Sep;129(2):401-14. doi: 10.1016/0042-6822(83)90179-4. PMID:6194613
Note on publication
The original publication investigates the antigenic structure and variation of canine parvovirus type-2, feline panleukopenia virus, and mink enteritis virus, employing monoclonal antibodies to identify antigenic variants within and between virus isolates from different animal species.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
