Cat. No.
MABL-607
Application
WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Burkholderia mallei, Burkholderia pseudomallei
Clone No.
CK2 (BP7 2C6)
From
Recombinant Antibody
Specificity
This antibody binds surface capsular antigens of Burkholderia mallei. B. mallei (BM), a host- adapted pathogen that does not normally persist in nature, causes glanders in horse. Some studies indicated that BM is highly infectious in humans by aerosol route. This antibody also cross reacts with surface capsular antigens of Burkholderia pseudomallei (BP), a causative agent of melioidosis, is a gram- negative, facultative anaerobic, motile bacillus commonly found in the soil and stagnant waters. BP infection is often due to either direct inoculation into wounds and skin abrasions or inhalation of contaminated materials. Recently, BP has been recognized as a major cause of community-acquired septicemia, resulting in significant mortality.
Alternative Names
surface capsule antigen; Burkholderia mallei; BM; glanders; Burkholderia pseudomallei; BP; Burkholderia spp; melioidosis; Whitmore’s disease
UniProt
Immunogen
The original antibody was generated by immunizing mice with various antigen preparations from either of B. mallei (American Type Culture Collection [ATCC] 23344) or B. pseudomallei (ATCC 23343).
Application Notes
This antibody can be used for the detection of heat killed or 0.02% SDS treated samples of BP and BP using an ELISA. This antibody recognized surface-capsule antigens with molecular sizes of 38 to 52 kDa, and 200 kDa in BM and weakly reactive to 14∼28, 200 kDa antigens in BP in a western blot (PMID: 21573027).
Antibody First Published
Yong-Kim et al. Production and characterization of chimeric monoclonal antibodies against Burkholderia pseudomallei and B. mallei using the DHFR expression system. PLoS One. 2011 May 9;6(5):e19867. PMID:21573027
Note on publication
Describes the generation and characterization of chimeric antibodies against Burkholderia pseudomallei and B. mallei.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
