Cat. No.
MABL-849
Application
antagonist, functional assay
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
108.5
From
Recombinant Antibody
Specificity
This antibody binds human CD277 (BTNA3A1). This protein plays an important role in T-cell activation and in the adaptive immune response. It regulates proliferation of activated T-cells and release of IFNG by activated T-cells. It is expressed on surface of T-cells, natural killer cells, dendritic cells and macrophages (at protein level). It is highly expressed in heart, pancreas and lung and moderately expressed in placenta, liver and muscle.
Alternative Names
BTN3A1; BTF5; BT3; BT3.1; Butyrophilin subfamily 3 member A1
UniProt
O00481
Immunogen
The original antibody was generated by immunizing BALB/c mice with soluble BT3.1-Ig fusion protein.
Application Notes
This antibody was used as an antagonist antibody to check if BTN3A can specifically enhance Vg9Vd2 TCR-mediated lysis of primary AML blasts using cytotoxic assays. This antibody was reported to significantly and drastically abrogate zoledronate (ZOL)-induced lysis (PMID： 27853633). This antibody can completely abrogate Vγ9Vδ2 T cell mediated lysis of tumors (PMID： 29296524).
Antibody First Published
Compte et al. Frontline： Characterization of BT3 molecules belonging to the B7 family expressed on immune cells. Eur J Immunol. 2004 Aug;34(8)：2089-99. PMID：15259006
Note on publication
This paper describes the characterization of BTN3A1 molecule expressed on immune cells using antibodies.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
