Cat. No.
MABL-852
Application
activation, Blocking, FA, in vivo, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Dog
Clone No.
1C6
From
Recombinant Antibody
Specificity
The antibody binds specifically to canine CD28, a self-surface receptor expressed by thymocytes and a subset of natural killer cells. CD28 acts as a receptor for CD80 and CD86, providing a costimulatory signal for the activation of T-cells and the production of interleukins, such as IL-6.
Alternative Names
TP44; Costimulatory molecule B7 receptor CD28; T-cell costimulatory molecule CD28
UniProt
Q9N0N8
Immunogen
Canine CD28.
Application Notes
This antibody exhibits an antagonistic activity, blocking mixed lymphocyte reactions and cytotoxic T lymphocyte-mediated killing. The use of this antibody to neutralize the CD26 signal following T-cell receptor activation is of clinical importance for transplantation therapy, as it has been shown to prevent graft rejection and graft-versus-host disease in the canine model of allogeneic hematopoietic cell transplantation (HCT). Interestingly, this antibody also exhibits agnostic activity; it was coated onto magnetic beads and used for the activation of canine peripheral blood lymphocytes (Szopa et al., 2021; PMID: 33679741).
Antibody First Published
Scott S. Graves, Diane M. Stone, Carol Loretz, Laura J. Peterson, Marina Lesnikova, Billanna Hwang, George E. Georges, Richard Nash and Rainer Storb. Antagonistic and agonistic anti-canine CD28 monoclonal antibodies: tools for allogeneic transplantation. Transplantation. 2011 Apr 27; 91(8): 833–840. PMID:21343872
Note on publication
Describes the role of CD28 in the activation of T cells via costimulatory signals, and the blockade of this signal using anti-CD28 monoclonal antibodies such as 1C6. Also demonstrates the advantages of using this method in comparison to cytotoxic T lymphocyte antigen 4 (CTLA-4)-Ig fusion protein by in vivo studies in the dog HCT model.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
