Cat. No.
MABL-862
Application
enhance, therapeutic, WB, ELISA, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
12G10
From
Recombinant Antibody
Specificity
This antibody recognises a ligand-induced binding site on the βA domain of β1 integrin, and thus reacts preferentially with α5β1 integrins in the ligand-activated state. Binding of this antibody alters the position of the α1 helix of β1 integrin, and results in further integrin activation.
Alternative Names
Integrin beta-1; Integrin β1 chain; Integrin β1; VLA-β chain; Glycoprotein Iia; GPIIA; Fibronectin receptor subunit beta; ITGB1; VLA-4 subunit beta; FNRB; MDF2; MSK12
UniProt
P05556
Immunogen
This antibody was raised by immunising BALB/C mice with purified β1 integrins from HT-1080 fibrosarcoma cells
Application Notes
This antibody reacts with β1 integrins in Western blotting (Mould et al, 1995). ELISA analysis has revealed the ability of this antibody to enhance the interaction between β1 integrin and its ligand, fibronectin (Mould et al, 1995). Indeed, activation of integrins by this antibody reduced the induction of osteosarcoma growth and inhibition of NF-κB caused by hERG1 knockdown in osteosarcoma cells (Zeng et al, 2016). This antibody has been used to specifically label ligand-bound cell surface β1 integrins both in immunohistochemical analysis (Izumi et al, 2015; Chao & Kunz, 2009), including in situ proximity ligation (PLA) analysis (Nevo et al, 2010), and in FACS analysis (Brandt et al, 2009).
Antibody First Published
Mould et al. Identification of a novel anti-integrin monoclonal antibody that recognises a ligand-induced binding site epitope on the beta 1 subunit. FEBS Lett. 1995 Apr 17;363(1- 2):118-22. PMID:7537221
Note on publication
Describes the original generation of this antibody, and its characterisation through Western blotting and ELISA experiments.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
