Cat. No.
MABL-955
Application
RIA, WB, FC
Isotype
Engineer antibody
Species Reactivity
Rat
Clone No.
OX-58
From
Recombinant Antibody
Specificity
This antibody recognises rat leukosialin, a major cell-surface sialoglycoprotein of thymocytes and T lymphocytes. It is also expressed on neutrophils, bone marrow stem cells and B-lineage cells after activation. The specificity of the leukosialin epitope is largely determined by linear protein sequence, as indicated by the reaction of this antibody with the extracellular domain of leukosialian expressed in an unglycosylated form in E. coli. However, the sensitivity of this antibody to neuraminidase treatment of leukosialin indicates that glycosylation can modify epitope conformation or flexibility.
Alternative Names
Leukosialin; Leukocyte sialoglycoprotein; Sialophorin; W3/13 antigen; OX 58; MRC OX 58
UniProt
P13838
Immunogen
This antibody was prepared by immunizing BALB/c mice with rat thymocyte leukosialin and thymocytes.
Application Notes
This antibody binds to rat leukosialin, and can be used to label thymocytes and T lymphocytes in the lymph node cell population in flow cytometry analysis. This antibody has also been used, along with other anti-CD43 MABs, in a Western blot analysis to localize leukosialin epitopes.
Antibody First Published
Cyster et al. The dimensions of the T lymphocyte glycoprotein leukosialin and identification of linear protein epitopes that can be modified by glycosylation. EMBO J. 1991 Apr;10(4):893-902. PMID:1706994
Note on publication
Describes the original use of this antibody, along with other anti-CD43 MAbs, in FC, WB and plate binding assay studies to determine the dimensions of the extracellular part of the leukosialin molecule, and to establish that the antigenic determinants recognized are largely based on linear protein sequences.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
