Cat. No.
MABL-966
Application
functional assay, therapeutic, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
F8:33
From
Recombinant Antibody
Specificity
This antibody is specific for CD44vRA, which is a CD44 derived from rheumatoid arthritis patients. CD44vRA contains an extra alanine. At higher concentration it will also bind to other isoforms of CD44. CD44 is cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment.
Alternative Names
CDw44; Epican; Extracellular matrix receptor III; ECMR-III; GP90 lymphocyte homing/adhesion receptor; HUTCH-I; Heparan sulfate proteoglycan; Hermes antigen; Hyaluronate receptor; Phagocytic glycoprotein 1; PGP-1
UniProt
P16070
Immunogen
The original mouse antibody was raised by immunizing C57BL/6 mice with soluble CD44vRA. CD44vRA is a form of CD44, containing an extra alanine, derived from rheumatoid arthritis patients.
Application Notes
To test the ability of the mouse version of this antibody to bind to CD44vRA, flow cytometry was preformed on Namalwa cells expressing different isoforms of CD44 (Golan et al, 2007; pmid:17383158). Further, it was used in ELISA preformed on different isotypes of the CD44 protein to assess the ability of this antibody to bind specific types of CD44; CD44vRA (CD44 from rheumatoid arthritis patients) was bound most strongly (Golan et al, 2007; pmid:17383158). Furthermore, flowcytometry was preformed to analyse apoptosis of synovial fluid cells. The mouse version of this antibody was used to induced apoptosis (Golan et al, 2007; pmid:17383158). It was administred to arthritic mice to reduce joint inflammation and induced apoptosis above the spontaneous level in the spleen of the mice (Golan et al, 2007; pmid:17383158). Transwell migration assay was preformed to assess the specificity of this antibody. It prevented namalwa-CD44vRA cells from crossing but did not prevent namalwa cells transfected with other forms of CD44 from crossing (US2011207678). Finally, flow cytometry was preformed with this antibody on synovial fluid cells and PBLs. The antibodies bound to the synovial fluid cells but not the PBLs from the same patient (Golan et al, 2007; pmid:
Antibody First Published
Golan et al. Expression of extra trinucleotide in CD44 variant of rheumatoid arthritis patients allows generation of disease-specific monoclonal antibody J Autoimmun. Mar-May 2007;28(2-3):99-113. PMID:17383158
Note on publication
Describes the generation of monoclonal antibodies (mAbs) that exclusively bind, at concentrations ranging from 2 to 100 microg/ml, to a modified CD44 variant (designated CD44vRA) expressed on synovial fluid cells from joints of rheumatoid arthritis (RA) patients.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
