Cat. No.
MABL-968
Application
Blocking, ICC, Western blot, ELISA, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
Hermes-3
From
Recombinant Antibody
Specificity
This antibody is specific for CD44 (gp90 epitope cluster III). Hermes-3 recognizes Hermes antigen homologues on monkey, goat, and sheep lymphocytes.
Alternative Names
CDw44; Epican; Extracellular matrix receptor III; ECMR-III; GP90 lymphocyte homing/adhesion receptor; HUTCH-I; Heparan sulfate proteoglycan; Hermes antigen; Hyaluronate receptor; Phagocytic glycoprotein 1; PGP-1; Phagocytic glycoprotein I; CD44/H-CAM antibody; Hermes 3
UniProt
P16070
Immunogen
This antibody was produced by immunizing BALB/c mice with purified Hermes-1 antigen from KCA, a human B lymphoblastoid line that binds preferentially to mucosal HEV.
Application Notes
This antibody has been used in IF, IHC (immunoperoxidase method), cross-blocking and WB studies to characterise the Hermes-defined antigens (Picker, 1989). This antibody is able to block lymphocyte binding to mucosal HEV, without affecting binding to HEV in peripheral lymph nodes and synovium (Jalkanen, 1987). H3-51 is cross-blocked by this antibody, indicating that the epitopes recognized by these two antibodies overlap (Picker, 1989).
Antibody First Published
Jalkanen et al. Lymphocyte recognition of high endothelium: antibodies to distinct epitopes of an 85-95-kD glycoprotein antigen differentially inhibit lymphocyte binding to lymph node, mucosal, or synovial endothelial cells The Journal of Cell Biology, 1987, Vol.105(2), p.983-990 PMID:2442176
Note on publication
Describes the original use of this antibody, along with others, to characterise the molecules involved of lymphocyte recognition of HEV.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
