Key features and details | |
Cat. No. | MABL-866 |
Name | Anti-CD3 mAbs |
Clone No. | AFD- CD3B219 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | in vitro, FC |
Species Reactivity | Human, monkey |
Basic Information | |
Specificity | The antibody is specific for CD3 epsilon. |
Alternative Name | T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e; SP34-2 |
UniProt | P07766 |
Immunogen | |
Application Notes | The antibody was used to construct different CD33 xCD3 bispecific antibodies (IgG4). In vitro T cell mediated cytotoxicity assays were performed using the bispecific antibody and the cytotoxicity was measured by flow cytometry. Ex vivo cytotoxicity assay showed the paired antibody promoted a dose-dependent reduction of total cytotoxicity that correlated with T cell activation after 48 hours (US20210040209A1). The antibody is considered a high affinity binder (Kd= 8mM) (US20210214440A1). The CD33×CD3 bispecific antibodies specifically induce cell cytotoxicity of MOLM-13 cells and CD33+ monocytes at 48 hr (US20200048349A1). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
