Cat. No.
MABL-1239
Application
crystallization, in vitro, in vivo, WB, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Bovine, chick, Rat, Human, Mouse
Clone No.
CIIC1
From
Recombinant Antibody
Specificity
The antibody binds the triple helical C1 epitope (GARGLT) of collagen type II.
Alternative Names
CII; Alpha-1 type II collagen; Collagen alpha-1(II) chain
UniProt
P02458
Immunogen
The original antibody was generated by immunizing DBA/1 mice with chick sternal type II collagen.
Application Notes
This anti-collagen type II antibody CIIC1 is an arthritogenic antibody, which induces arthritis in mice (Holmdahl et al., 1986; PMID: 2421741 and Nandakumar et al., 2003; PMID: 12884302). The antibody specificity towards collagen type II was assayed in an ELISA. The antibody was selected for in vivo injection experiments in naive DBA/1 male mice, at no time was any arthritis detected macroscopically after treatment with 0.5, 2 or 5 mg of antibody per animal, however development of synovitis was noticed (Holmdahl et al., 1986; PMID: 2421741). The structure of the Fab fragment was determined by X-ray crystallography. The specificity of the full length CIIC1 antibody and the FV fragment was confirmed by ELISA analysis. Further investigation by using both the full length antibody and a single chain Fv fragment in ELISA experiments revealed that the antibody bound also to other IgG1, IgG2a and IgG2b anti-collagen antibodies (Uysal et al., 2008; PMID: 18241923). The antibody detected collagene type II by western blot analysis (Burkhardt et al., 2005; PMID: 15832289). In vitro studies demonstrated that the antibody together with M2139 antibody suppressed the self-assembly of CII into fibrils (Croxford et al., 2010; PMID: 20662051). This antibody was used for immunohistochemistry on cartilaginous tissue (Vickers et al., 2010; PMID: 20225321).
Antibody First Published
Holmdahl et al. Characterization of the antibody response in mice with type II collagen-induced arthritis, using monoclonal anti-type II collagen antibodies. Arthritis Rheum. 1986 Mar;29(3):400-10. PMID:2421741
Note on publication
The paper describes the generation and characterization of twenty monoclonal antibodies reactive with type II collagen.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
