![Anti-Aquaporin 4 [rAb-53] (MABL-3602)](/uploads/allimg/20260123/1-260123092AQY.png)
Cat. No.
MABL-3602
Application
cell-based ELISA, functional assay, IF
Isotype
Human IgG1
Species Reactivity
Human
Clone No.
rAb-53
From
Recombinant Antibody
Specificity
This antibody binds an extracellular conformation specific epitope on the human aquaporin 4 (AQP4), a water channel expressed on astrocytes throughout the CNS and in skeletal muscle and some epithelial cells outside of the CNS. It plays an important role in brain water homeostasis and in glymphatic solute transport. Neuromyelitis optica spectrum disorder (NMOSD) is an inflammatory disease of the central nervous system (CNS) that is associated with demyelination and neurological deficits in optic nerve, spinal cord and brainstem. Most NMOSD patients are seropositive for IgG autoantibodies ( NMO-IgG) directed against extracellular epitopes of aquaporin-4 (AQP4). Prevention of the binding of NMO-IgG, therefore, may alleviate the disease.
Alternative Names
AQP4; AQP-4; AQmab; aquaporumab; Aquaporin-4; MIWC; WCH4; Mercurial-insensitive water channel; rAb53; Aquaporumab; AQmabAM
UniProt
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Immunogen
The original antibody rAb-53 was generated from the PCR products obtained from the human B cell clones isolated from CSF plasma of an neuromyelitis optica (NMO-IgG) seropositive patient using a dual vector transient transfection system. The affinity matured version was generated by using saturation mutagenesis.
Application Notes
This antibody is an affinity matured version of rAb-53 (Aquaporumab). The binding of this antibody to the extracellular region of AQP4 in CHO cells stably expressing the M23 isoform of human AQP4 was done using a cell-based ELISA. When AQP4-expressing cells were incubated with affinity matured rAb-53 and human complement antibody it produced complement-dependent cytotoxicity (CDC) with EC50 ~ 82 ng/ml. The Fc silent version of this antibody with LALA mutations, also called as AQmabAM bound to AQP4 in cell cultures with Kd ~ 18 ng/ml (~ 0.12 nM). AQmabAM prevented CDC produced by sera from eight NMO patients with IC50 ranging from 40–80 ng/ml, and similarly prevented antibody-dependent cellular cytotoxicity (ADCC). Mechanistic studies demonstrated that AQmabAM blocked binding of serum NMO autoantibodies to AQP4. This antibody can be used for the visualization of AQP4 on the surface of cells using immunofluorescence (PMID: 31654702).
Antibody First Published
Duan et al. Affinity-matured ‘aquaporumab’ anti-aquaporin-4 antibody for therapy of seropositive neuromyelitis optica spectrum disorders. Neuropharmacology. 2020 Jan 1:162:107827. PMID:31654702
Note on publication
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Size
1mg
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
