Cat. No.
MABL-1274
Application
WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
P20
From
Recombinant Antibody
Specificity
This antibody reacts with human C reactive protein, which is a pentameric protein found in blood plasma. It is an acute phase reactive protein of hepatic origin. It is an important defense molecule for the body and its plasma concentration increases sharply during tissue damage, inflammation and bacterial infection. It promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. It can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
Alternative Names
C reactive protein; PTX1; C-reactive protein
UniProt
P02741
Immunogen
The original antibody was generated by immunizing BALB/c mice with C-reactive protein extracted from human plasma.
Application Notes
Indirect ELISA was used for the primary screening of the hybridomas showing selective binding for human CRP. Horseradish peroxide labelled scFv P20 was used for the identification of natural CRP extracted from denatured and non-denatured blood in a western blot assay. The combination of P02 and P20 antibody was also used in the double antibody sandwich detection system consisting of P20 as the coating antibody and P02 as the labeled antibody to be used in the colloidal gold detection platform for the detection of natural CRP. These antibodies were also used in the preparation of human C-reactive protein colloidal gold test card (CN106596976).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
