Cat. No.
MABL-1276
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Bacillus thuringiensis (Bt) bacteria
Clone No.
3H9
From
Recombinant Antibody
Specificity
The antibody binds to Cry1 toxins (Cry1Aa, Cry1Ab, Cry1Ac, Cry1B, Cry1C, Cry1F). The antibody does not cross react with Cry2A and vip3.
Alternative Names
Cry1 toxins; Cry1Aa; Cry1Ab; Cry1Ac; Cry1B; Cry1C; Cry1F; Pesticidal crystal protein Cry1Aa; 133 kDa crystal protein; Crystaline entomocidal protoxin; Insecticidal delta- endotoxin CryIA(a); Pesticidal crystal protein Cry1Fa; 134 kDa crystal protein; Insecticidal delta-endotoxin CryIF(a); Pesticidal crystal protein Cry1Ab; 130 kDa crystal protein; Insecticidal delta-endotoxin CryIA(b); Pesticidal crystal protein Cry1Ac;133 kDa crystal protein; Insecticidal delta-endotoxin CryIA(c)
UniProt
Q03746; P0A366; P0A370; P05068; D0EI56; Q93T75
Immunogen
The original antibody was generated by immunizing Balb/c mice with a mixture of six Cry1 toxins (Cry1Aa, Cry1Ab, Cry1Ac, Cry1B, Cry1C, and Cry1F) followed by antibody library construction and phage display-based selection.
Application Notes
The specificity of the scFv antibody for the six Cry1 toxins was confirmed by ELISA. The scFv fragment was used to establish the double antibody sandwich enzyme-linked immunosorbent assay method (DAS-ELISA) for detecting six Cry1 toxins (Cry1Aa, Cry1Ab, Cry1Ac, Cry1B, Cry1C, and Cry1F). The lowest detectable limits (LOD) and the lowest quantitative limits (LOQ) were 3.14–11.07 and 8.22–39.44 ng mL−1 , respectively, with the correlation coefficient higher than 0.997. The average recoveries of Cry1 toxins from spiked rice leaf samples were ranged from 84 to 95%, with coefficient of variation (CV) less than 8.2%, showing good accuracy for the multi-residue determination of six Cry1 toxins in agricultural samples (Dong et al., 2018; PMID: 29484477).
Antibody First Published
Dong et al. Screening for single-chain variable fragment antibodies against multiple Cry1 toxins from an immunized mouse phage display antibody library. Appl Microbiol Biotechnol. 2018 Apr;102(7)：3363-3374. PMID：29484477
Note on publication
The paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
