Cat. No.
MABL-1305
Application
inhibit, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
V44D7
From
Recombinant Antibody
Specificity
This antibody binds human CXCR3. This protein acts as a receptor for C-X-C chemokine CXCL9, CXCL10 and CXCL11 and mediates the proliferation, survival and angiogenic activity of human mesangial cells (HMC) through a heterotrimeric G-protein signaling pathway. Isoform 2 human mediates the proliferation, survival and angiogenic activity of microvascular endothelial cells (HMVEC) through a cAMP- mediated signaling pathway. Some of the disorders associated with CXCR3 signaling include diabetes mellitus type 1 and psoriasis.
Alternative Names
CD183; CXC-R3; CXCR-3; CKR-L2; IP-10 receptor; GPR9; C-X-C chemokine receptor type 3; G protein-coupled receptor 9; Interferon-inducible protein 10 receptor
UniProt
P49682
Immunogen
The original antibody was generated by immunizing BALB/c mice with CXCR-3 expressing NSO cells. The humanized version of the antibody was created by grafting CDRs of the original murine antibody onto human framework regions.
Application Notes
The binding characterization of this antibody to human CXCR3 was done using competitive ELISA. This antibody is capable of inhibiting binding of 125I-labeled IP-10 and -I-Tac to primary Th1 cells. This antibody can be used for the study of expression of CXCR3 on the surface of Th1 cells using flow cytometry. This antibody also inhibited Th1 cell migration mediated by CXCR3 chemokines (US8435522).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
