Cat. No.
MABL-1309
Application
functional assay, IP, WB, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
427aB1
From
Recombinant Antibody
Specificity
This antibody is specific for human CXCR4. It binds to both the monomers and homodimers. CXCR4 is a receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. Involved in the AKT signaling cascade. Plays a role in regulation of cell migration, e.g. during wound healing.
Alternative Names
C-X-C chemokine receptor type 4; CXC-R4; CXCR-4; FB22; Fusin; Fusin; LCR1; Leukocyte-derived seven transmembrane domain receptor; LESTR; Lipopolysaccharide-associated protein 3; LAP-3; LPS-associated protein 3; NPYRL; Stromal cell-derived factor 1 receptor; SDF-1 receptor; CD184
UniProt
P61073
Immunogen
The original mouse antibody was raised by immunizing balb/c mice with recombinant NIH3T3- CXCR4 cells and peptides corresponding to CXCR4 extracellular N-term and loops.
Application Notes
ELISA was preformed on N-terminal CXCR4 peptide conjugated to BSA with the mouse version of this antibody todemonstrate that the antibody binds to CXCR4 (WO2013017566). To determine the charataristics of this antibody, immunoprecipitation was preformed on NIH3T3-CXCR4 cells using the mouse version (WO2013017566). Western blot was also shown to work on proteins that were immunoprecipitated using the mouse version of this antibody (WO2013017566). IH3T3, NIH3T3-hCXCR4 transfected cells, MDA-MB-231 , Hela, HT-29 and U937 cancer cell lines were used for flow cytometry using the mouse version of this antibody to characterize binding patternrns of this clone (WO2013017566). To test the competition of binding to human CXCR4, the mouse version of this antibody and clone 515H7 were tested using flow cytometery on NIH3T3-hCXCR4 cells (WO2013017566). The influence of the mouse version of this antibody on the CXCR4 protein was tested using BRET analysis (WO2013017566).
Antibody First Published
WO2013017566
Note on publication
Describes the generation and characterization of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
