Cat. No.
MABL-1337
Application
WB, FC, IHC
Isotype
Engineer antibody
Species Reactivity
canine, Chicken, Hamster, Rabbit, Rat, Swine, Zebrafish, Human, Mouse
Clone No.
RGE53
From
Recombinant Antibody
Specificity
The antibody reacts exclusively with Cytokeratin 18 which is present in glandular epithelial cells of the digestive, respiratory, and urogenital tracts, endocrine and exocrine cells and mesothelial cells, as well as adenocarcinomas originating from them.
Alternative Names
K18; CK-18; Keratin K18; Keratin type I cytoskeletal 18; Cell proliferation- inducing gene 46 protein; Cytokeratin-18; Keratin-18
UniProt
P05783
Immunogen
The original antibody was generated by immunizing a BALB/c mouse with a cytoskeletal preparation of HeLa cells.
Application Notes
The antibody recognized glandular epithelial cells from female breast, digestive, respiratory, and urogenital tracts, endocrine and exocrine tissues, and mesothelial cells. Further, the antibody could distinguish adenocarcinomas and mesotheliomas, which stain positively in the indirect immunofluorescence technique (Ramaekers et al., 1983; PMID： 6193333). Further, the antibody produced fibrillar staining patterns in cultured HeLa cells, in cultured human hepatoma cells, F9 mouse teratocarcinoma cells and in a human colon carcinoma cell line (WiDr-218). The antibody detected Cytokeratin 18 in HeLa cells and cultured human hepatoma cells by western blot analysis (Ramaekers et al., 1983; PMID： 6350235). The antibody reacted with glandular and ductal epithelia, including those of the gastrointestinal tract, prostate, and kidney and with hepatocytes and mesothelium in immunohistochemistry (Ramaekers et al., 1990; PMID： 1690512).
Antibody First Published
Ramaekers et al. Antibodies to intermediate filament proteins in the immunohistochemical identification of human tumors： an overview Histochem J. 1983 Jul;15(7)：691-713. PMID：6350235
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
