Cat. No.
MABL-1339
Application
WB, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
ABM58058-41A2-PU
From
Recombinant Antibody
Specificity
This antibody is capable of binding human cytokeratin 19. Cytokeratins are intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. Cytokeratin 19 is involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Alternative Names
CK19; CK-19; CK 19; KRT19; K19; Keratin, type I cytoskeletal 19; Cytokeratin-19; Keratin-19; CGMCC No. 17489; A53-B/A2.26
UniProt
P08727
Immunogen
The original antibody was generated by immunizing 4-6 weeks old female BLAB/c with human cytokeratin conjugated with KLH in equal volume of Freund's incomplete adjuvant.
Application Notes
The binding characterization of this antibody was done using ELISA. This antibody is also capable of detecting human cytokeratin 19 in a western blot assay. The affinity constant of the original IgG2a antibody was determined to be 7.68X10^(9) M^(-1). This antibody was also used in the immunohistochemical staining of thyroid papillary cancer, colon cancer and gastric cancer tissues (CN111410690). The immunohistochemical staining pattern of this antibody is similar to another commercially available antibody A53-B/A2.26.
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
