Cat. No.
MABL-1348
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Naja nigricollis
Clone No.
TPL0027_01_A2
From
Recombinant Antibody
Specificity
TPL0027_01_A2 antibody recognizes cytotoxin 1, which is one of the major toxins in the venom of the black-necked spitting cobra (Naja nigricollis).
Alternative Names
CTX1; CX1; Cardiotoxin F8; Cardiotoxin-I; CTX-I; Toxin CM-6; toxin CX1
UniProt
Immunogen
The antibody was raised using IONTAS phage display library, a naïve human scFv library consisting of 4.0 · 10^10 unique antibody fragments. Through phage display selection, scFv-presenting phages were allowed to bind a toxin, phages with unspecific binding were washed away, and the rest were eluted through enzymatic digestion. These were then amplified and used for the following round. In total three rounds of selections were completed leaving the best binders behind.
Application Notes
TPL0027_01_A2 antibody has a very high affinity to the cytotoxin 1. It was demonstrated by normalized ELISA, which circumvents the limitation of a simple ELISA by removing the expression variable, normalizing the amount of scFv in each well. The antibody's high affinity was confirmed by its ability to bind the target protein in a venom fraction even at 1:32,000 dilution (3.125 · 10^-2) µg/mL (Jürgensen et al., 2019 [poster]). It is recommended for the research on the antitoxin against Naja nigricollis snakebites.
Antibody First Published
Jürgensen et al. Harnessing monoclonal antibodies for development of a specific treatment against Naja nigricollis envenoming. 10.13140/RG.2.2.24771.14884.
Note on publication
This poster describes the generation and characterization of this anti-cytotoxin 1 antibody among other antibodies directed against the venom toxins of Naja nigricollis.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
