Cat. No.
MABL-1367
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Fusarium species
Clone No.
AbDON01
From
Recombinant Antibody
Specificity
This antibody is specific for mycotoxin deoxynivalenol (DON). This antibody also shows reactivity with other trichothecene mycotoxins like 15-AC-deoxynivalenol, HT-2 toxin, T-2 triol, nivalenol (NIV) and T-2 toxin/tri-acetyl-DON (Tri-Ac-DON) but it does not react with 3-acetyl-deoxynivalenol (3-Ac- DON), Fusarenone-X (FX), trichothecin, diacetoxyscirpenol (DAS), roridin A, and verrucarol. . Deoxynivalenol belongs to the trichothecene class of mycotoxins, generated by several Fusarium fungi. Deoxynivalenol (DON), along with 3-acetyl-deoxynivalenol (3-ADON) and 15-acetyldeoxynivalenol (15-ADON), occur in grains and cereal products and is often hazardous to humans and livestock. Biochemically, deoxynivalenol is an inhibitor of protein synthesis and has hemolytic activity, causing apoptosis (cytotoxicity) and immunotoxicity to eukaryotic organisms. In animals, deoxynivalenol can cause anorexia and vomiting (PMID: 8896360; 16420629), and it can also cause human skin irritation, hemorrhaging, hematological changes, lymphocyte blastogenesis impairment, radiomimetic effects and apoptosis (PMID: 8896360; 16326049). In plants, deoxynivalenol is a virulence factor in the pathogenesis of G. zeae on wheat (PMID: 16420629).
Alternative Names
DON; Mycotoxin; Vomitoxin, Fusarium mycotoxin; ChEBI: 10022; 1.3G2
UniProt
Immunogen
The original antibody was generated by cloning the variable light and heavy chain genes of the anti-DOM hybridoma were assembled into the scFv gene using a linker sequence (Gly4Ser)3 by over- lapping PCR. PCR amplified products were purified, treated with SfiI and NotI and cloned into phagemid expression pDisplay-2TM . The plasmid was used to transform E.coli HB2151 host cells and the soluble scFv was generated.
Application Notes
The binding characterization of the scFv antibody and the whole IgG1 antibody was done using non-competitive indirect ELISA. To check the cross reactivity of the scFv version and whole IgG version with other members of trichothecene mycotoxins, competitive direct ELISA and biolayer interferometry (BLI) was used (Maragos et al., 2012).
Antibody First Published
Maragos et al. Production and characterization of a single chain variable fragment (scFv) against the mycotoxin deoxynivalenol. Food and Agricultural Immunology (2012), 23:1, 51-67
Note on publication
Describes the generation of a single chain antibody fragment (scFv) antibody having reactivity towards deoxynivalenol.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
