Cat. No.
MABL-1373
Application
IP, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Rat, Human, Mouse
Clone No.
7G6
From
Recombinant Antibody
Specificity
This antibody recognizes desmocollin-2 (also known as Desmocollin-3). Desmocollins (next to desmogleins) are the main desmosomal transmembrane proteins.The expression of desmocollin-2 varies in the basal and suprabasal layers.
Alternative Names
Desmocollin; Desmocollin-2; Cadherin family member 2; Desmocollin-3; Desmosomal glycoprotein II; Desmosomal glycoprotein III; Cadherin family member 3; Dsc2/3; Dsc2; Dsc3; DG2; DG3
UniProt
Q02487
Immunogen
This antibody was raised by immunizing mice with a MBP-tagged recombinant protein corresponding to the extracellular domain of human Desmocollin-2.
Application Notes
The antibody 7G6 is recommended for the detection and analysis of desmocollin-2 (desmocollin-3) via various methods, such as immunohistochemistry, Western blot and others. For instance, the antibody was used to label desmocollin-2 in primary human keratinocytes via immunohistochemistry (Franke et al., 2006). Similarly, another group detected desmocollin-2 in A431 cells (Wahl et al., 2005). The antibody 7G6 was also utilized in the assessment of expression levels of desmocollin-2 in SHEEC and KYSE510 cells via Western blot (Fang et al., 2014).
Antibody First Published
Parker et al. Desmosomal glycoproteins II and III. Cadherin-like junctional molecules generated by alternative splicing. J Biol Chem. 1991 Jun 5;266(16):10438-45 PMID: 2037591
Note on publication
This article describes the anti-desmocollin-2 antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
