Cat. No.
MABL-1374
Application
IP, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
AK23
From
Recombinant Antibody
Specificity
This antibody binds mouse Dsg3 and also shows reactivity for human Dsg3. It does not show any affinity towards Dsg1. AK23 recognizes a calcium-dependent conformational epitope on Dsg3, which consisted of the V3, K7, P8, and D59 Dsg3-specific residues that formed the adhesive interface between juxtaposed Dsg.
Alternative Names
DSG3; PVA; CDHF6; Desmoglein-3; 130 kDa pemphigus vulgaris antigen; Cadherin family member 6
UniProt
P32926
Immunogen
The original antibody was generated by immunizing Dsg3 knockout mice with recombinant mouse Dsg3-His in complete Freund's adjuvant subcutaneously.
Application Notes
This antibody can be used to study the blister formation and complex interaction that occur in autoimmune blistering disease like Pemphigus vulgaris (PV). Pemphigus vulgaris (PV) is an epidermal blistering disorder caused by antibodies directed against the desmosomal cadherin desmoglein-3 (Dsg3). AK23 was reported to induce pemphigus vulgaris (PV) blisters in neonatal mice. The binding affinity of this antibody for human and mouse desmoglein 3 was determined using ELISA. This antibody was also used in the live keratinocyte staining of mouse PAM212 cell line and indirect immunofluorescent staining of mouse oral mucuous membrance, mouse liver and lower parts of the human epidermis. Immunoprecipitaton was used for the determination of the epitope of the antibody (PMID: 12574390). This antibody was also used in the study of alteration in desmosome morphology (PMID: 23226536).
Antibody First Published
Tsunoda et al. Induction of pemphigus phenotype by a mouse monoclonal antibody against the amino-terminal adhesive interface of desmoglein 3. J Immunol. 2003 Feb 15;170(4):2170-8. PMID: 12574390
Note on publication
Describes the generation of this antibody in pemphigus vulgaris mouse model and examines its pathogenic activity in the induction of blister formation.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
