Cat. No.
MABL-1375
Application
IP, WB, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
6D8
From
Recombinant Antibody
Specificity
This antibody recognizes human desmoglein-2. This clone does not cross-react with Desmoglein 1 or Desmoglein 3. The epitope is within the extracellular domain (EC3/EC4) of Desmoglein 2/DSG2. Desmoglein-2 is part of a cadherin protein family and is involved in the creation of desmosomes.
Alternative Names
Cadherin family member 5; Desmoglein 2; HDGC; DSG2; 3G132
UniProt
Q14126
Immunogen
This antibody was raised by immunizing mice with a recombinant human full-length DSG2 protein.
Application Notes
The antibody 6D8 has been used successfully to identify and analyse desmoglein-2 via various methods. For instance, it was utilized to label desmoglein 2 in melanoma tissue samples via immunohistochemistry to investiagete expression pattern of DSG2 in this cancer (Tan et al., 2016; pmid: 27340778). Furthermore, this antibody was also used to label DSG2 during flow cytometric analysis of cells (Tan et al., 2016; pmid: 27340778). The antibody 6D8 has also been applied in a Western blot detection of desmogleins in transfected A-431D cells (Wahl, 2002; pmid: 11991815). Finally, this antibody immunoprecipitated desmoglein-a from A-431 cell extracts prepared in 0.1% Empigen BB (Wahl, 2002; pmid: 11991815).
Antibody First Published
Wahl et al. Plakoglobin domains that define its association with the desmosomal cadherins and the classical cadherins: identification of unique and shared domains. J Cell Sci. 1996 May;109 ( Pt 5): 1143-54. PMID: 8743961
Note on publication
This article describes the characterization of the antibody 6D8.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
