Cat. No.
MABL-1376
Application
dot blot, IP, WB, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
5G11
From
Recombinant Antibody
Specificity
This antibody recognizes desmoglein 3. It does not cross-react with desmoglein 1 or desmoglein 2. Desmoglein-3 is a calcium binding membrane protein that is localized in desmosome cellular junctions and interacts with plaque proteins and intermediate filaments at cell-cell adhesion points.
Alternative Names
PVA; Cadherin family member 6; desmoglein 3; 130 kDa pemphigus vulgaris antigen; DSG3
UniProt
P32926
Immunogen
This antibody was raised by immunizing Balb/c mice with a recombinant protein corresponding to human desmoglein-3.
Application Notes
This antibody can be used for detection and analysis of desmoglein-3 via various methods. For instance, 5G11 was utilized todetect desmoglein-3 in human scalp hair follicles and epidermal inclusions via immunohistochemistry (Wu et al., 2003). Another group successfully used this antibody to identify desmoglein-3 via Western blot (Mao et al., 2017; pmid: 28469076). The antibody 5G11 was also used to detect DSG3 in cells via immunofluorescence (Chen et al., 2013; pmid: 23737966).
Antibody First Published
Wahl Generation of monoclonal antibodies specific for desmoglein family members. Hybrid Hybridomics. 2002 Feb;21(1):37-44. PMID: 11991815
Note on publication
This article describes the generation and characterization of the antibody 5G11.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
