Cat. No.
MABL-1377
Application
ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
F779
From
Recombinant Antibody
Specificity
This antibody binds human desmoglein-3 between amino acids 1-161 and does not cross react with desmoglein-1. It is a component of intercellular desmosome junctions. It is involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. Pemphigus vulgaris (PV) is a potentially lethal skin disease in which epidermal blisters occur as the result of the loss of cell-cell adhesion caused by the action of autoantibodies against desmoglein 3. It is characterized by suprabasal blistering of skin and mucous membranes.
Alternative Names
DSG3; PVA; CDHF6; Desmoglein 3; 130 kDa pemphigus vulgaris antigen; Cadherin family member 6
UniProt
P32926
Immunogen
The original antibody was generated from the peripheral blood of four patients with active untreated pemphigus vulgaris (PV) using heterohybridoma techniques.
Application Notes
This antibody can be used for the determination of desmoglein-3 using ELISA. The binding affinity of IgG1 version of this antibody for human desmoglein-3 was determined using surface plasmon resonance and was found to be Kd = 1.77 nM (PMID: 24942562). This antibody was also used in the direct and indirect immunofluorescence staining of frozen human skin sections (PMID: 24942562; 27304671) The IgG1 and IgG4 variants of this antibody induce loss of cell surface Dsg3 in primary human epidermal keratinocytes and suprabasal blisters in human skin (PMID: 27304671). This antibody is pathogenic in nature and caused suprabasal blisters in human skin at comparable doses of 25 μg and 50 μg (PMID: 27304671).
Antibody First Published
Cho et al. Shared VH1-46 gene usage by pemphigus vulgaris autoantibodies indicates common humoral immune responses among patients. Nat Commun. 2014 Jun 19;5:4167. PMID: 24942562
Note on publication
This paper identifies VH1-46 autoantibody gene as a common gene expressed in patients with pemphigus vulgaris.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
